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DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit |
 | | | | DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit |
| | | Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. | |
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Prices are in US dollars and valid only for US orders.
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- Reliable results with mRNA, total RNA, viral RNA and in vitro transcribed RNA
- Multiple targets can be detected from one cDNA synthesis reaction
- Convenient, optimized 2X mixes for both cDNA synthesis and qPCR
- Broad Dynamic Range - up to 1 μg of RNA
- Licensed for PCR*
Description:
Finnzymes' DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit and DyNAmo Capillary SYBR Green 2-Step qRT-PCR Kit enable reproducible detection and quantification of RNA with great sensitivity and specificity. The kits includes all the necessary reagents for cDNA synthesis and following SYBR Green qPCR. The reverse transcriptase in the kits is M-MuLV RNase H+, which provides higher sensitivity to qPCR than RNase H- reverse transcriptases. The RNase H activity in the RT enzyme facilitates annealing of PCR primers to the cDNA by degrading the RNA template from the RNA-cDNA hybrid before the PCR step. The performance of the qPCR step is based on a hot start version of a modified Thermus brockianus (Tbr) DNA polymerase. A non-specific DNA binding domain has been fused to the polymerase, which lends physical stability to the polymerase-DNA complex. DyNAmo SYBR Green 2-step qRT-PCR Kit is compatible with all block-based real-time qPCR instruments and DyNAmo Capillary SYBR Green 2-Step qRT-PCR Kit is engineered for capillary based instruments.
Broad dynamic range:
Amplification and standard curves below (Figures 1a and 1b) show the wide dynamic range and excellent linearity of the DyNAmo SYBR Green 2-Step qRT-PCR kits.
Figures 1a and 1b: Human HeLa cell total RNA amounts varying from 10 pg up to 1 ug were reverse transcribed using random priming. In the following qPCR 140 bp Calmodulin 1 fragment was amplified with cDNA specific primers. The cDNA synthesis reactions were done in 20 µl volumes, from which 1/14 part was transferred to qPCR reactions. Reactions were set up on the DNA Engine Opticon® system (MJ Research, Inc).
Advantages:- Broad dynamic range: up to 1 µg of RNA.
- Reliable results with different RNA sources: mRNA, total RNA, viral RNA and in vitro transcribed RNA.
- Flexible: two-step system, all priming options.
- Optimized 2X mixes for both cDNA synthesis and qPCR.
- Specific and sensitive: uniquely engineered Tbr DNA polymerase.
- Convenient to use: 2X mixes and short protocols.
Kit Components:
Master Mix (2X)
RT Reaction Buffer (2X)
M-MuLV RNase H+ Reverse Transcriptase (includes RNase Inhibitor)
Oligo(dT)15 primer (100 ng/μl)
Random Hexamers (300 ng/μl)
ROX Passive Reference Dye (50X)
Storage Conditions
Storage Temperature:
-20°C
Notes
General notes:- Both DyNAmo SYBR Green 2-Step qRT-PCR kits are stable for six months from the date of packaging when stored and handled properly.
FAQs
- What applications are the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits recommended for?
- What are the advantages to using the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits?
- What reverse transcriptase is used in the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits?
- What instruments can the DyNAmo™ SYBR® Green 2-Step qRT-PCR kits be used with?
- Are Finnzymes' DNA Polymerases licensed for PCR?
Companion Products
DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ Capillary SYBR® Green qPCR Kit
DyNAmo™ cDNA Synthesis Kit
DyNAmo™ Flash Probe qPCR Kit
DyNAmo™ Flash SYBR® Green qPCR Kit
DyNAmo™ HS SYBR® Green qPCR Kit
DyNAmo™ Probe 2-Step qRT-PCR Kit
DyNAmo™ Probe qPCR Kit
DyNAmo™ SYBR® Green qPCR Kit
DyNAmo™ SYBR® Green qPCR Kit With ROX™ Passive Reference Dye
Legal
Licenses/Patents/Disclaimers:
* PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.
Notice to purchaser: Limited license. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. SYBR® Green I stain is covered by patents belonging to Molecular Probes and is provided under license from Molecular Probes, Inc. for use only in combination with real-time PCR.
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