Uncomparable Sensitivity - reproducible detection of low-copy number templates
Broad Dynamic Range - up to eight orders of magnitude starting even from a single copy of the target gene
Specificity — hot start polymerase minimizes primer dsimer formation and non-specific products
Description: DyNAmo™ Capillary SYBR® Green qPCR Kit is a ready-to-use 2X master mix for highly specific quantitative PCR optimized for instruments using capillary tubes (e.g. LightCycler™). DyNAmo Capillary 2X Master Mix contains all reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The 2X master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA binding domain that lends physical stability to the polymerase-DNA complex.
The recommended kit for the users of block based instruments is DyNAmo HS SYBR Green qPCR Kit.
Specificity and Sensitivity: Amplification of a difficult genomic template below demonstrates the excellent performance of the DyNAmo Capillary SYBR Green qPCR Kit. As low as three copies of the template were reliably detected, and the melting curve shows that no unspecific products were amplified.
Figures 1 and 2: A 481 bp amplicon of a beta actin gene was amplified with DyNAmo Capillary SYBR Green Kit using starting copy numbers ranging from 3 to 1 x 105. Reactions were set up on the LightCycler™ System (Roche). The cycling parameters were the following: Advantages:
Specificity: DyNAmo Capillary SYBR Green Master Mix minimizes the formation of unwanted primer-dimers and extension of non-specifically bound primers.
Sensitivity: Optimized master mix allows reproducible detection of low-copy number templates.
Early detection: The modified polymerase and optimized reaction conditions in DyNAmo Capillary SYBR Green qPCR Kit enable efficient amplification.
Convenience: 2X master mix, one tube only. No addition of enzyme or MgCl2.
The master mix provides 3 mM MgCl2 in final reaction concentration.
Kit Components: Master Mix (2X)
Storage Conditions Storage Temperature: -20°C
Notes General notes:
DyNAmo Capillary SYBR Green qPCR Kit is stable for six months from the date of packaging when stored and handled properly.
Legal Licenses/Patents/Disclaimers: * PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.
Notice to purchaser: Limited license. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. SYBR® Green I stain is covered by patents belonging to Molecular Probes and is provided under license from Molecular Probes, Inc. for use only in combination with real-time PCR.
