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DyNAmo™ Flash SYBR® Green qPCR Kit |
 | | | | DyNAmo™ Flash SYBR® Green qPCR Kit |
| | | Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. | |
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Prices are in US dollars and valid only for US orders.
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- Speed — cycling times are dramatically reduced with no compromises in performance
- Robustness — modified DNA polymerase enhances processivity and yield
- Sensitivity — high signal intensity results in reliable quantitation and early C(t) values
- Specificity — hot start modification of DNA polymerase reduces non-specific amplification
Description:
Finnzymes´ novel DyNAmo™ Flash SYBR® Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than other SYBR Green kits without compromising the qPCR performance. A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYBR Green qPCR Kit gives reliable quantitation and early C(t) values. Due to the high signal intensity, DyNAmo Flash SYBR Green qPCR provides an excellent signal-to-noise ratio.
Figure 1: Fast real-time qPCR This is a comparison of qPCR cycling times between SYBR Green qPCR kits from different suppliers. All protocols were conducted as recommended by the manufacturers and set up on the DNA Engine Opticon® 2 system (Bio-Rad Laboratories, Inc). With the new DyNAmo Flash SYBR Green qPCR Kit, the cycling time can be reduced by as much as 92 min compared to other SYBR Green kits.
Figure 2: Efficient amplification and high signal intensity Amplification of a 141 bp cDNA amplicon fron human calmodulin gene with DyNAmo Flash SYBR Green qPCR Kit (A; combined annealing and extension 15 s) and a kit from another supplier (B; annealing 30 s + extension 30 s). Reaction and cycling protocols recommended by the suppliers were followed. DyNAmo Flash SYBR Green qPCR Kit delivers efficient amplification and higher signal intensity (note different fluorescence scales in A and B).
Kit Components:
Master Mix (2X)
ROX Passive Reference Dye (50X)
Storage Conditions
Storage Temperature:
-20°C
Notes
General notes:- Upon arrival, store all kit components at -20 °C. When using the 2x master mix, the leftover thawed mix can be refrozen and stored at -20 °C without affecting the performance of the kit. The kit is stable for six months from the date of packaging when stored and handled properly.
Companion Products
DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ Capillary SYBR® Green qPCR Kit
DyNAmo™ Flash Probe qPCR Kit
DyNAmo™ HS SYBR® Green qPCR Kit
DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ SYBR® Green qPCR Kit
DyNAmo™ SYBR® Green qPCR Kit With ROX™ Passive Reference Dye
Legal
Licenses/Patents/Disclaimers:
* PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.
Notice to purchaser: Limited license. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. SYBR® Green I stain is covered by patents belonging to Molecular Probes and is provided under license from Molecular Probes, Inc. for use only in combination with real-time PCR.
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