 |
|
 |
|
|
| Home >
Products >
Polymerases >
qPCR Products >
DyNAmo™ HS SYBR® Green qPCR Kit |
 | | | | DyNAmo™ HS SYBR® Green qPCR Kit |
| | | Developed & Manufactured By Finnzymes Oy. Distributed by New England Biolabs, Inc. | |
| | |
|
|
|
 |
|
Prices are in US dollars and valid only for US orders.
|
- Licensed for PCR*
- Superior Specificity - hot start version further minimizes primer-dimer formation and non-specific amplification
- Uncomparable Sensitivity - reproducible detection of low-copy number templates
- Broad Dynamic Range - up to eight orders of magnitude starting even from a single copy of the target gene
Description:
DyNAmo™ HS SYBR® Green qPCR Kit is a ready-to-use 2X master mix for highly specific quantitative PCR. The master mix contains all reagents needed for qPCR. Only template DNA and PCR primers need to be added by the user. The 2X master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA binding domain that lends physical stability to the polymerase-DNA complex.
ROX passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.
DyNAmo HS SYBR Green qPCR Kit is compatible with all block-based real-time qPCR instruments. The recommended kit for the users of capillary based instruments is DyNAmo Capillary SYBR Green qPCR Kit.
Specificity and Sensitivity:
Amplification and melting curves below (Figures 1 and 2) show that DyNAmo HS SYBR Green qPCR Kit has an excellent performance over a broad range of template copy numbers, and amplifies a specific product even from a difficult template.
Figures 1a and 1b: A 335 bp PCR product was amplified from Lambda DNA. Reactions were set up on the DNA Engine Opticon system (MJ Research, Inc). Reaction volumes of 20 µl were used with starting template copy number ranging from 5 to 50,000,000 copies.
Figures 2a-2c: A 481 bp amplicon of a beta actin gene was amplified with DyNAmo HS Kit. Reactions were set up on the DNA Engine Opticon system (MJ Research, Inc). Reaction volumes of 20 µl were used with starting template amount ranging from 0.035 ng to 200 ng. The melting curve shows that the DyNAmo HS Kit has amplified the fragment with excellent specificity.
Advantages:- Specificity: DyNAmo HS SYBR Green Master Mix minimizes the formation of unwanted primer-dimers and extension of non-specifically bound primers during reaction setup.
- Sensitivity: Optimized master mix allows reproducible detection of low-copy number templates.
- Broad dynamic range: DyNAmo HS SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude.
- Convenience: 2X master mix, one tube only. ROX passive reference dye provided in a separate vial.
Kit Components:
Master Mix (2X)
ROX Passive Reference Dye (50X)
Notes
General notes:- The DyNAmo HS SYBR Green qPCR Kit is shipped in gel ice.
- Upon arrival store all kit components at -20°C.
- The DyNAmo HS SYBR Green Kit is stable for six months from the date of packaging when stored and handled properly.
- After use, the leftover thawed 2X master mix can be refrozen and stored at -20°C.
FAQs
- What is the enzyme in DyNAmo™ qPCR kits?
- What applications is DyNAmo™ HS SYBR® Green qPCR Kit recommended for?
- What are the advantages to using DyNAmo™ HS SYBR® Green qPCR Kit?
- Do I need the ROX™ passive reference dye when I use DyNAmo™ HS SYBR® Green qPCR Kit?
- What is the difference between DyNAmo™ SYBR® Green qPCR kits (#F-400/F-400R/F-420) and DyNAmo™ HS SYBR® Green qPCR Kit (#F-410)?
- Can DyNAmo™ HS SYBR® Green qPCR Kit be used with any instrument?
- I have non-specific products after amplification using DyNAmo™ HS SYBR® Green qPCR Kit.
- I have no product after amplification using a DyNAmo™ qPCR kit.
- Can I clone the PCR product after amplifying with DyNAmo™ qPCR Kits?
- Does the enzyme in the DyNAmo™ qPCR kits add 3' A to the PCR product to make it compatible with TA cloning?
- What should I do if there are non-specific products that are not primer dimers in my no template control when using a DyNAmo™ qPCR kit?
- What should I do if there are bubbles in my qPCR reactions before cycling using a DyNAmo™ qPCR kit?
- Does the polymerase in DyNAmo™ qPCR kits have 5' → 3' exonuclease activity?
- Does the polymerase in DyNAmo™ qPCR kits have proofreading capabilities (3' → 5' exonuclease activity)?
- Is the enzyme in my DyNAmo™ qPCR kit a hot-start polymerase?
- At what temperature should the DyNAmo™ kit be stored?
- Are Finnzymes' DNA Polymerases licensed for PCR?
Companion Products
DyNAmo™ Capillary SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ Capillary SYBR® Green qPCR Kit
DyNAmo™ cDNA Synthesis Kit
DyNAmo™ Flash Probe qPCR Kit
DyNAmo™ Flash SYBR® Green qPCR Kit
DyNAmo™ Probe 2-Step qRT-PCR Kit
DyNAmo™ Probe qPCR Kit
DyNAmo™ SYBR® Green 2-Step qRT-PCR Kit
DyNAmo™ SYBR® Green qPCR Kit
DyNAmo™ SYBR® Green qPCR Kit With ROX™ Passive Reference Dye
Legal
Licenses/Patents/Disclaimers:
* PCR license notice: These products are sold under licensing arrangements of Finnzymes Oy with F. Hoffman-La Roche LTD. The purchase of these products is accompanied by a limited license to use them in the Polymerase Chain Reaction (PCR) process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front fee, either by payment to Applied Biosystems or as purchased, i.e. an authorized thermal cycler.
Notice to purchaser: Limited license. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product. SYBR® Green I stain is covered by patents belonging to Molecular Probes and is provided under license from Molecular Probes, Inc. for use only in combination with real-time PCR.
|
|
|
 |
|
|
