 |
|
 |
|
|
| Home >
Products >
Protein Tools >
Phage Display >
Ph.D.™ Peptide Display Cloning System |
 | | | | Ph.D.™ Peptide Display Cloning System |
| | | | |
 |
|
Prices are in US dollars and valid only for US orders.
|
Description:
The Ph.D.™ Peptide Display Cloning System facilitates the display of custom peptide libraries on the surface of bacteriophage M13 as coat protein fusions, creating a physical linkage between each displayed peptide and its encoding DNA sequence. Peptide ligands for a variety of targets can then be selected by the straightforward method of panning. The supplied display vector M13KE is an M13 derivative with cloning sites engineered for N-terminal pIII fusion, resulting in a valency of 5 displayed peptides per virion. The use of a phage vector, rather than a phagemid, simplifies the intermediate amplification steps, since neither antibiotic selection nor helper phage superinfection are required. Since displayed proteins longer than 20-30 amino acids have a deleterious effect on the infectivity function of pIII in phage vectors, this vector is suitable only for the display of short peptides. Included with the cloning vector is an insert extension primer as well as a detailed protocol for cloning a peptide library into M13KE.
* This product contains 20 μg of M13KE gIII Cloning Vector and 2150 pmol of M13KE Extension Primer.
Kit Components:
M13KE Extension Primer
pMAL-p4E Vector
Storage Conditions
Storage Temperature:
-20°C
References
- Zwick, M.B. et al. (1998) Anal Biochem, 264, 87-97.
- Noren, K.A. and Noren, C.J. (2001) Methods, 23, 169-178.
Reagents Sold Separately
pMAL-p4E Vector
Companion Products
M13KE Phage
Ph.D.™-12 Phage Display Peptide Library Kit
Ph.D.™-7 Phage Display Peptide Library Kit
Ph.D.™-C7C Phage Display Peptide Library Kit
pMAL-pIII Vector
|
|
|
 |
|
|
