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MBP5-paramyosin-ΔSal
Cloned At NEBRecombinant Source
Catalog # Size Concentration Price Qty  
E8052S 100 μg 5 mg/ml $59.00
Prices are in US dollars and valid only for US orders.
Download:MSDS PDF


Description:
MBP5-paramyosin-ΔSal is a maltose binding fusion protein (MBP) containing a Factor Xa site between the MBP and paramyosin domains. It can be used as a control substrate for Factor Xa cleavage. Its predicted molecular weight is 69,710 daltons, and after Factor Xa cleavage it produces two fragments of 42,510 and 27,218 daltons.

Source:
MBP5-paramyosin-ΔSal is prepared from an E. coli strain bearing a modified pMAL-c5X vector, using the Protein Fusion and Purification System. The modified pMAL-c5X vector contains a fragment of the D. immitis paramyosin gene inserted between the XmnI site and the SalI site.


Reaction & Storage Conditions



Concentration:
5 mg/ml

Storage Conditions:
20 mM Tris-HCl
0.2 M NaCl
1 mM DTT
50% Glycerol


Storage Temperature:
-20°C


References


  1. Riggs, P.D.  (1990) In F.M. Ausebel et al. (Eds.), Current Protocols in Molecular Biology, pp. 16.6.1-16.6.12. 
  2. Steel, C.S. et al.  (1990) J. Immunol, 145, 3917-3923.


Legal


Research Use Assurance:
The buyer/user has a non-exclusive license to use the vector for Research Purposes Only. Commercial use of this vector requires a license from New England Biolabs, Inc.

U.S. Patent No. 5,643,758

Appln. No. WO2007/120809

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