New England Biolabs
To access your account, log in or register.
Products Technical Reference Customer Service My NEB Account
Contact NEB About Us Site Map Request a Catalog OEM at NEB International Orders Freezer Program Quick Order
Related Information
Protocols for Anti-MBP Monoclonal Antibody HRP Conjugated
FAQs for Protein Expression and Purification
Technical Reference for Protein Expression and Purification
Favorite Tools
Enzyme Finder
NEBcutter
NEBuffer Chart
Double Digest Finder
PCR Selection Tool
Isoschizomers
DNA Sequences
and Maps
REBASE
Discounts, Limited Offers and Value Purchases
Anti-MBP Monoclonal Antibody HRP Conjugated
Catalog # Size Concentration Price Qty  
E8038L 0.25 ml 1 mg/ml $540.00
E8038S 0.05 ml 1 mg/ml $135.00
Prices are in US dollars and valid only for US orders.
Download:MSDS PDF


Description:
Anti-MBP Monoclonal Antibody (HRP conjugated) is a murine anti-maltose binding protein antibody, isotype IgG2a. It is covalently linked to horseradish peroxidase and purified (1,2).

Recommended Working Dilution for Westerns:
1/2000–1/5000

Antibody Type: Monoclonal Isotype: Mouse Isotype IgG2a

Storage Conditions


Storage Conditions:
10 mM HEPES
150 mM NaCl
50% Glycerol
pH 7.5 @ 25°C

Storage Temperature:
-20°C


Notes


General notes:
  1. Store at -20°C undiluted. May be stored at 4°C diluted in buffer containing 1 mM NaN3 or an equivalent antimicrobial agent.

Protocols
spacer
spacer
Protocols for Anti-MBP Monoclonal Antibody HRP Conjugated


Quality Control for Current Lot


Quality control values for a specific lot can be found on the datacard which accompanies each product.

Quality Assurance Statement:
In an ELISA assay, a dilution of 1/2000 gives a signal of at least 20% of the maximum signal using concentrated antibody (detected with OPD as the substrate). The same 1/2000 dilution gives a strong signal when used to detect maltose-binding protein in Western blots developed with a variety of detection systems. This antibody does not cross-react with other E. coli proteins.


References


  1. Nakane, P. K. and Kawaoi, A. (1974) J. Histochem. Cytochem. 22, 1084 - 1091.
  2. Boorsma, D. M. and Streefkerk, J. G. (1979) J. Histochem. Cytochem. 24, 481 - 486.

New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company.
NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201
Privacy, Limitations, Warranty, Disclaimer, Copyright & Trademark