 | | | | Chitin Magnetic Beads | | | Available in bulk quantites, please contact bulks@neb.com | |
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Prices are in US dollars and valid only for US orders.
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Description:
An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD) (1). Chitin beads have been prepared having a magnetite core. This permits the magnetic isolation of CBD-fusion proteins from cell culture supernatants; after which, the matrix can be regenerated without loss of binding capacity. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that interact with the immobilized fusion protein.
Support Matrix: approximately 50-70 μm paramagnetic microparticles.
Supplied As: slurry (50%) containing 20% ethanol
Properties
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| CBD fusion protein | | 0.4 mg/ml | | |
Storage Conditions
Storage Conditions:
155 mM NaCl
0.02% sodium azide
0.05% Tween-20
1.06 mM KH2PO4
2.97 mM Na2HPO4
pH 7.2 @ 25°C
Storage Temperature:
4°C
Do not freeze.
Notes
General notes:- Efficiency of elution can be checked by eluting any protein that remains bound to the chitin magnetic beads with 50 µl of SDS-PAGE gel loading buffer and running 15 µl on a denaturing protein gel.
References
- Chong, S., Mersha, F. B., Comb, D. G., Scott, M. E., Landry, D., Vence, L. M., Perler, F. B., Benner, J., Kucera, R. B., Hirvonen, C. A., Pelletier, J. J., Paulus, H. and Xu, M.-Q. (1997) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein-splicing element. Gene, 192, 271-281.
Companion Products
6-Tube Magnetic Separation Rack
Chitin Beads
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