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Protocols for Amylose Magnetic Beads FAQs for Gene Expression and Protein Purification Technical Reference for Gene Expression and Protein Purification Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE 6-Tube Magnetic Separation Rack Amylose Resin Amylose Resin High Flow Anti-MBP Magnetic Beads

Home > Products > Gene Expression and Protein Purification > Magnetic Matrices and Racks > Amylose Magnetic Beads Amylose Magnetic Beads Catalog # Size Concentration Price Qty   E8035S 25 mg 10 mg/ml $150.00 Prices are in US dollars and valid only for US orders. Download: Technical Bulletin|MSDS PDF Description: An affinity matrix for the small-scale isolation and purification of MBP-fusion proteins. Amylose is covalently coupled to a paramagnetic particle through a linkage that is stable and leak resistant over a wide pH range. This permits the isolation of MBP-fusion proteins from cell culture supernatants. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that may interact with the immobilized fusion protein. Supplied as a 10 mg/ml suspension in water containing 20% ethanol. Support Matrix: 10 μm superparamagnetic microparticles. Properties Ligand Binding Capacity MBP-fusion protein 10 μg Storage Conditions Storage Conditions: 10 mg/ml suspension in water 20% Ethanol Storage Temperature: 4°C Do not freeze. Notes General notes: Efficiency of elution can be checked by eluting any protein that remains bound to the Amylose Magnetic Beads with 50 µl of SDS-PAGE gel loading buffer and running 15 µl on denaturing protein gel. Companion Products 6-Tube Magnetic Separation Rack Amylose Resin Amylose Resin High Flow Anti-MBP Magnetic Beads