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Anti-GLuc Antibody |
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Prices are in US dollars and valid only for US orders.
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Description:
Anti-GLuc Antibody is an IgG fraction from rabbit serum immunized with recombinant Gaussia luciferase (GLuc). It can detect GLuc secreted from cells or in cell lysates.
Western blot with anti-GLuc Antibody of proteins expressed in HEK-293 cells. GLuc and a non-secreted GLuc-fusion protein (~50 kDa) were detected in cell culture media and cell lysates. Lane 1: Untransfected cells; Lanes 2&3: GLuc-fusion; Lane 4: GLuc. The gel was blotted onto PVDF membrane; GLuc Antibody was diluted 1:5000 and detected with a chemiluminescent substrate and HRP-coupled anti-rabbit secondary antibody.
Specificity/Cross-reactivity: Tested in extracts from mammalian cells transfected with Gaussia luciferase expression plasmids.
Storage Conditions
Storage Conditions:
0.154 mM NaCl
50% Glycerol
0.01 mM phosphate-buffered saline
Storage Temperature:
-20°C
Notes
Usage notes:- Recommended Dilution: 1:2000 - 1:5000
Protocols
Protocols for Anti-GLuc Antibody
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
The Anti-GLuc Antibody is used in a Western analysis detecting the presence of Gaussia Luficerase in the growth medium and in the cell lysate after transfection of cells (mammalian) with a GLuc-expressing vector.
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New
England Biolabs, Inc. is an ISO 9001 and ISO
14001 Certified Company.
NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201 |
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