To access your account, log in or register.	shopping cart | log in

FAQs for Gene Expression and Protein Purification Technical Reference for Gene Expression and Protein Purification Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Gene Expression and Protein Purification > E. coli - Companion Products > Amylose Resin High Flow Amylose Resin High Flow Catalog # Size Concentration Price Qty   E8022L 100 ml   $600.00 E8022S 15 ml   $150.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: Amylose Resin High Flow is a cross-linked affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP). This rigid matrix can be used in automated chromatography systems. Column Hardware Pressure Limit: 0.5 MPa or 75 psi Maximum recommended Flow Velocity: 300 cm/hour For a 1.6 cm column diameter: 10 ml/minute For a 2.5 cm column diameter: 25 ml/minute Column Buffer: 20 mM Tris-HCl (pH 7.4) 0.2 M NaCl 1 mM EDTA Optional: 1 mM DTT or10 mM b-mercaptoethanol Properties Ligand Binding Capacity MBP2* -paramyosin ΔSal fusion protein 7 mg/ml Storage Conditions Storage Conditions: 20% Ethanol Storage Temperature: 4°C Notes General notes: Store at 4°C. After use, resin should be stored in column buffer plus 0.02% sodium azide or 20% ethanol. Usage notes: Amylose Resin column should be washed with 5 volumes of column buffer before each use. For optimum performance, load crude extract at < 60 cm/hour. When regenerating the column at 4°C, please note that 0.1% SDS can precipitate at that temperature. It is therefore recommended that the SDS solution be stored at room temperature until needed. The resin may be generated up to five times. For a complete affinity purification protocol, download the pMAL Protein Fusion and Purification System technical bulletin (NEB #E8000) from www.neb.com. Regeneration: The packed resin may be regenerated by the following wash sequence: Water 3 - column volumes, 0.1% SDS - 3 column volumes, Water - 1 column volume, Column Buffer - 5 column volumes. Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. Quality Assurance Statement: Crude extract from E. coli containing a plasmid that expresses a MBP2* -paramyosinDSal fusion protein is passed over a 1 ml column at 4°C. The column is then washed with 10 column volumes of column buffer. The protein is eluted with column buffer plus 10 mM maltose. Electrophoresis on a 4-20% SDS-PAGE gel results in a single band.