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FAQs for Gene Expression and Protein Purification Technical Reference for Gene Expression and Protein Purification Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Gene Expression and Protein Purification > E. coli - Companion Products > Amylose Resin Amylose Resin Catalog # Size Concentration Price Qty   E8021L 100 ml   $395.00 E8021S 15 ml   $110.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: An affinity matrix used for the isolation of proteins fused to maltose-binding protein. It is a composite amylose/agarose bead. Supplied As: Liquid Properties Ligand Binding Capacity Maltose-binding Protein (MBP) 3 mg/ml MBP-β-galactosidase 6 mg/ml Storage Conditions Storage Conditions: 20% Ethanol Storage Temperature: 4°C Notes General notes: Although the column is washed at 4°C, 0.1% SDS will eventually precipitate at that temperature. It is therefore recommended that the SDS solution be stored at room temperature until needed. The resin may be generated up to five times. The used resin should be stored in column buffer +0.02% sodium azide. Usage notes: Quantitative Analysis: Crude extract from E. coli containing a plasmid that expresses a maltose-binding protein-β-galactosidase fusion protein (100,000 units) is passed over a 1 ml column at 4°C. The column is then washed with 10 column volumes of 20 mM Tris-HCl (pH 7.4), 0.2 M NaCl, 10 mM β-mercaptoethanol, 1 mM EDTA. The protein is eluted with above buffer plus 10 mM maltose. Electrophoresis on a 4-20% SDS-PAGE gel results in a single band. Regeneration: The packed resin may be regenerated by the following wash sequence: Water 3 - column volumes, 0.1% SDS - 3 column volumes, Water - 1 column volume, Column Buffer - 5 column volumes.