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Anti-PB1 Peptide Antibody |
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Prices are in US dollars and valid only for US orders.
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Description:
Serum from rabbits immunized with PB1 peptide (CTRSRHSSYPNEYEEDEEMEEEL). Anti-PB1 Peptide Antibody is used to detect the PB1 peptide sequence after its ligation to a carrier protein (1-3). Control Peptide, PB1 (NEB #S6605) is a control peptide in the Peptide-Carrier™ Kit (NEB #E6600); it is derived from mouse Bad protein (MW, 2862 Da).
Suggested Working Dilution: 1:2000
Performance: In a Western blot, a dilution of 1:2000 gives a strong signal when used to detect fusion protein of PB1 peptide ligated to Carrier Protein 39 (Western) (NEB #E6602).
Figure 1: Western blot analysis of antiserum raised against Control Peptide, PB1. PB1 was synthesized with a N-terminal cysteine (CTRSRHSSYPNEYEEDEEMEEEL; MW, 2862 Da) and ligated to Carrier Protein 39 (Western) (NEB #E6602). The reaction times and temperatures are indicated. The samples were electrophoresed on a 12% SDS polyacrylamide gel and transferred to nitrocellulose. Anti-PB1 Peptide Antibody (NEB #E6608) was used as the primary antibody and HRP-linked anti-rabbit antibody was used as the secondary antibody. Lanes 1 and 5 are controls (C) which contain only Carrier Protein 39 (Western).
Specificity/Cross-reactivity: Tested by Western blot analysis. Reacts specifically with PB1 peptide.
Storage Conditions
Storage Conditions:
10 mM HEPES
150 mM NaCl
50% Glycerol
pH 7.5 @ 25°C
Storage Temperature:
-20°C
Notes
Usage notes:- 75-90% of the Carrier Protein 39 (Western) is ligated when the purified peptide is used at a final peptide concentration of 0.5-1 mM.
Use of certain unpurified peptides may result in lower ligation efficiency (10-50%). This will still result in a positive signal on Western blot analysis.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Analysis:
Carrier Protein 39 (Western) (NEB #E6602) is ligated to the Control Peptide PB1 (NEB #S6605) containing an N-terminal cysteine (CTRSRHSSYPNEYEEDEEMEEEL; MW, 2862 Da) in the presence of 1X Carrier Protein Reaction Buffer for 60 minutes at room temperature (1). A positive signal is detected on Western blot analysis with Anti-PB1 Antibody (1:2000) (NEB #E6608).
References
- Ghosh, I., Sun, L., Evans, T.C., Jr. and Xu, M.-Q. (2004) J. Immunol. Methods, In press.
- Xu, J., Sun, L., Ghosh, I. and Xu, M.-Q. (2004) Biotechniques, 36, 976-981.
- Sun, L., Rush, J., Ghosh, I., Maunus, J. and Xu, M.-Q. (2004) Biotechniques, 37.
Companion Products
Carrier Protein (CP) Reaction Buffer
Carrier Protein 27 (Array)
Carrier Protein 27 (Western)
Carrier Protein 39 (Array)
Carrier Protein 39 (Western)
Control Peptide, PB1
Peptide-Carrier™ Kit
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