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LongAmp™ Taq PCR Kit |
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Prices are in US dollars and valid only for US orders.
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Description:
The LongAmp™ Taq PCR Kit features LongAmp Taq DNA Polymerase, which is a unique blend of Taq and Deep VentR® DNA Polymerases. The 3´→5´ exonuclease activity of Deep VentR Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). LongAmp Taq DNA Polymerase offers up to six fold higher fidelity than Taq Polymerase alone. The LongAmp Taq PCR Kit is supplied with LongAmp Taq Reaction Buffer, which is optimized to give highest PCR yields up to 40 kb. Mg-free buffer is also supplied for situations when complete control over the final magnesium concentration is required to optimize amplification. The kit can generate a wide range of PCR products, up to 40 kb from lambda DNA and 30 kb from human genomic DNA.
LongAmp Taq DNA Polymerase supplied in: 100 mM KCl, 10 mM Tris-HCl (pH 7.4@ 25°C), 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween 20, 0.5% NP-40 and 50% Glycerol.
1X LongAmp™ Taq DNA Polymerase Reaction Buffer:
60 mM Tris-SO4 (pH 9.0 @ 25°C)
20 mM (NH4)2SO4
2 mM MgSO4
3% glycerol
0.06% NP-40
0.05% Tween-20
Applications:Advantages:- Offers up to 6-fold higher fidelity than Taq DNA Polymerase alone
- Allows for robust amplification of PCR products up to 40 kb from low complexity templates and up to 30 kb from difficult templates, like human genomic DNA
Kit Components:
dNTP Mix (10 mM)
LongAmp™ Taq (Mg-free) Reaction Buffer Pack (5X)
LongAmp™ Taq DNA Polymerase (2,500 units/ml)
LongAmp™ Taq Reaction Buffer Pack (5X)
MgSO4 (100 mM)
Nuclease Free dH2O
Storage Conditions
Storage Temperature:
-20°C
Notes
General notes:- LongAmp Taq Reaction Buffer supplied in: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 0.1% Triton X-100 and 50% Glycerol.
- LongAmp Taq DNA Polymerase (2,500 units/ml):
A blend of Taq DNA Polymerase and Deep Vent Polymerase
FAQs
- What is the recommended enzyme amount?
- What is the fidelity of the LongAmp Taq DNA Polymerase over Taq?
- Can the extension step be carried out at 72°C?
- What is the extension rate?
- Why is the product a smear when visualized on an agarose gel?
- Can the kit be used to amplify GC-rich amplicons?
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Long Amplicon PCR::
LongAmp Taq PCR Kit is tested for the ability to generate a 40 kb fragment from lambda DNA and a 30 kb fragment from human genomic DNA.
Amplification of specific sequences from human genomic DNA using LongAmp Taq DNA Polymerase. Amplicon sizes are indicated below the gel. Marker M is NEB 1 kb DNA Ladder (NEB #N3232).
References
- Barnes, W.M. (1994) Proc. Natl. Acad. Sci., 91, 2216-2220.
- Foord, O.S. and Rose, E.A. (1994) PCR Methods Appl., 3, 149-161.
- Sun, Y. et al. (1993) Biotechniques, 15, 372-374.
- Sarkar, G. et al. (1990) Nucleic Acids Res., 18, 7465.
Reagents Sold Separately
LongAmp™ Taq (Mg-free) Reaction Buffer Pack
LongAmp™ Taq DNA Polymerase
LongAmp™ Taq Reaction Buffer Pack
Companion Products
LongAmp™ Taq 2X Master Mix
Legal
Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
The purchase of this product conveys to the purchaser only the limited, non-transferable right to use the purchased quantity of the product for the purchaser's own research by the purchaser only under the following U.S. patent claims and foreign counterpart patent claims: U.S. Patent Number 5,436,149 (claims 6-16). No rights are granted to the purchaser to sell, modify for resale or otherwise transfer this product, either alone or as a component of another product, to any third party. Takara Bio reserves all other rights, and this product may not be used in any manner other than as provided herein. For information on obtaining a license to use this product for purposes other than research, please contact Takara Bio Inc., Seta 3-4-1, Otsu, Shiga 520-2193, Japan (Fax +81-77-453-9254).
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