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Taq PCR Kit |
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Prices are in US dollars and valid only for US orders.
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- Robust and reliable reactions
- Tolerates a wide range of templates
- Incorporates dUTP, dITP and fluorescently-labeled nucleotides
- Exceptional value in terms of cost per unit
Description:
The Taq PCR Kit contains a supply of recombinant, highly purified Taq DNA Polymerase, PCR-qualified buffer solutions, deoxynucleotides and a broad range, pre-mixed, ready-to-load DNA marker to perform 200 PCR reactions.
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a non-processive 5´→ 3´ polymerase activity and a double-strand specific 5´→ 3´ exonuclease activity. It is the enzyme most widely used in PCR (1).
The Taq PCR Kit is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems. Mg-free buffer is also supplied for situations when complete control over the final magnesium concentration is required to optimize amplification.
Background:
The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification. PCR amplifies specific DNA sequences exponentially by using multiple cycles of a three-step process. First, the double-stranded DNA template is denatured at a high temperature. Sequence-specific primers are then annealed to sites flanking the target sequence. A thermostable DNA polymerase, such as Taq DNA Polymerase, extends the annealed primers, thereby doubling the amount of the original DNA sequence. This newly synthesized product then becomes an additional template for subsequent cycles of amplification. These three steps are repeated for 20 to 30 cycles, resulting in a 105-109 fold increase in DNA concentration (1, 2).
Kit Components:
Taq DNA Polymerase with Standard Taq Buffer
Deoxynucleotide Solution Mix
MgCl2
Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb)
Standard Taq (Mg-free) Reaction Buffer Pack (10X)
Storage Conditions
Storage Temperature:
-20°C
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
3´→5´ Exonuclease Activity:
Incubation of 20 units of Taq DNA Polymerase in 20 µl of a 10 nM solution of a fluorescent 5´-FAM labeled oligonucleotide for 30 minutes at 37°C and 75°C showed no detectable 3´→5´ degradation when analyzed by high resolution denaturing PAGE.
Endonuclease Activity:
Incubation of 20 units of Taq DNA Polymerase with 1 µg ΦX174 RF I DNA for 4 hours at 37°C and 75°C in a 50 µl reaction resulted in <10% conversion to RF II as determined by agarose gel electrophoresis.
PCR
Taq PCR Kits are qualified for use in PCR by demonstrating the ability to amplify both a 500 bp single-copy human gene and an 8 kb Lambda amplicon in a 25 cycle reaction.
Reaction Buffers
All NEB 10X reaction buffers are free of detectable nucleases.
Deoxynucleotide Solution
Nucleotide solutions are certified free of nucleases and phosphatases. Functional purity is determined by chain termination sequencing reactions.
References
- Saiki, R.K. et al. (1985) Science, 230, 1350-1354.
- Scharf, St.J. et al. (1986) Science, 233, 1076-1078.
- Chien et al. (1976) J. Bact., 127, 1550-1557.
Reagents Sold Separately
Taq DNA Polymerase with Standard Taq Buffer
Deoxynucleotide Solution Mix
Quick-Load® 2-Log DNA Ladder(0.1-10.0 kb)
Standard Taq (Mg-free) Reaction Buffer Pack
Companion Products
Taq 2X Master Mix
Taq DNA Polymerase Diluent
Taq DNA Polymerase with ThermoPol Buffer
Taq PCR Kit with Controls
Deoxynucleotide Solution Set
Quick-Load® Taq 2X Master Mix
ThermoPol II (Mg-free) Reaction Buffer Pack
ThermoPol Reaction Buffer
Legal
Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
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