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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Strains > Strains > E. coli K12 ER2420/pBeloBAC11 E. coli K12 ER2420/pBeloBAC11 Limit of 1 per order Catalog # Size Concentration Price Qty   E4154S 200 μl   $0.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A suspension of E. coli strain ER2420 containing the plasmid pBeloBAC11 which has been grown in rich medium and brought to 50% glycerol. ER2420 background: EcoK r- m- McrBC- Dam+ Dcm+. Genotype: F- ara-14 leu fhuA2 Δ(gpt-proA)62 lacY1 glnV44 galK2 rpsL20 xyl-5 mtl-1 Δ(mcrC-mrr)HB101 Recommended Growth Medium: LB Growth Temperature: 37°C Growth Note: The plasmid pBeloBAC11 carries a gene encoding resistance to chloramphenicol (cam). To maintain the plasmid, cells should be grown with 15-20 µg/ml cam. Strain Properties dam/dcm Methylation: dam+ , dcm+ Protease-deficient: No Laclq: No Drug Resistance: cam Storage Conditions Storage Temperature: -20°C For long term storage (>30 days), store at -70°C. Notes The blue color of non-recombinant pBeloBac11 clones on indicator plates is not as intense as that obtained with multicopy vectors that also use α-complementation of β-galactosidase. We recommend incubation for 20-24 hours at 37°C to achieve the full color development necessary to evaluate transformation efficiency and guide selection of white colonies. Color development will intensify further if the plates are subsequently stored at 4°C. Usage notes: Rubidium Chloride Method: RbCl Transformation Protocol References Shizuya, H. et al. (1992) Proc. Natl. Acad. Sci. USA, 89, 8794-8797. Mori, H. et al. (1986) J. Mol. Biol., 192, 1-15. Imber, R., Low, R.L. and Ray, D.S. (1983) Proc. Natl. Acad. Sci. USA, 80, 7132-7136. Disque-Kochem, C. et al. (1986) Mol. Gen. Genet., 202, 132-135. Komori, H. et al. (1999) EMBO J., 18, 4597-4607. Lane, D. et al. (1986) Nucl. Acids Res., 14, 9713-9728.