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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Strains > Strains > E. coli K12 POP2136/pFOS1 E. coli K12 POP2136/pFOS1 Limit of 1 per order Catalog # Size Concentration Price Qty   E4153S 200 μl   $0.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A suspension of E. coli strain POP2136 containing the plasmid pFOS1 which has been grown in rich medium and brought to 50% glycerol. POP2136 background: EcoK r- m+ McrBC+ Dam+ Dcm+. Genotype: F- glnV44 hsdR17 endA1 thi-1 aroB mal- cI857 lambdaPR tetR Recommended Growth Medium: LB Growth Temperature: 30°C Growth Note: Grow cells at 30°C. The plasmid is unstable at 37°C. The plasmid pFOS1 carries a gene encoding resistance to ampicillin (amp) and a gene encoding resistance to chloramphenicol (cam). To maintain the plasmid, cells should be grown with 100 µg/ml amp and 15-20 µg/ml cam. Strain Properties dam/dcm Methylation: dam+ , dcm+ Protease-deficient: No Laclq: No Drug Resistance: cam, tet, amp Storage Conditions Storage Temperature: -20°C For long term storage (>30 days), store at -70°C. Notes Usage notes: Rubidium Chloride Method: RbCl Transformation Protocol References Shizuya, H. et al. (1992) Proc. Natl. Acad. Sci. USA, 89, 8794-8797. Mori, H. et al. (1986) J. Mol. Biol., 192, 1-15. Imber, R., Low, R.L. and Ray, D.S. (1983) Proc. Natl. Acad. Sci. USA, 80, 7132-7136. Disque-Kochem, C. et al. (1986) Mol. Gen. Genet., 202, 132-135. Komori, H. et al. (1999) EMBO J., 18, 4597-4607. Lane, D. et al. (1986) Nucl. Acids Res., 14, 9713-9728.