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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Strains > Strains > E. coli K12 ER2420/pACYC184 E. coli K12 ER2420/pACYC184 Limit of 1 per order Catalog # Size Concentration Price Qty   E4152S 200 μl   $0.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A suspension of E. coli strain ER2420 containing the plasmid pACYC184 which has been grown in rich medium and brought to 50% glycerol. ER2420 background: EcoK r- m- McrBC- Mrr- Dam+ Dcm+. Genotype: F- ara-14 leu fhuA2 Δ(gpt-proA)62 lacY1 glnV44 galK2 rpsL20 xyl-5 mtl-1 Δ(mcrC-mrr)HB101 Recommended Growth Medium: LB Growth Temperature: 37°C Growth Note: The plasmid pACYC184 carries a gene encoding resistance to tetracycline (tet) and a gene encoding resistance to chloramphenicol (chloramphenicol acetyltransferase). To maintain the plasmid, cells should be grown with 25-50 µg/ml of tet and 35-50 µg/ml of cam. The plasmid, which is present at a low copy number, cannot be amplified using a standard chloramphenicol procedure but can be amplified using spectinomycin (50 µg/ml of growth media). Strain Properties dam/dcm Methylation: dam+ , dcm+ Protease-deficient: No Laclq: No Drug Resistance: tet, cam Storage Conditions Storage Temperature: -20°C For long term storage (>30 days), store at -70°C. Notes Usage notes: Rubidium Chloride Method: RbCl Transformation Protocol References Chang, A. and Cohen, S. (1978) J. Bacteriol., 134, 1141-1156. Rose, R.E. (1988) Nucl. Acids Res., 16, 355.