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E. coli K12 CJ236 |
 | | | | E. coli K12 CJ236 | | | Limit of 1 per order | |
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Description:
A suspension of E. coli strain CJ236 which has been grown in LB medium and brought to 50% glycerol.
Genotype: FΔ(HindIII)::cat (Tra+ Pil+ CamR)/ ung-1 relA1 dut-1 thi-1 spoT1 mcrA
Recommended Growth Medium: LB
Growth Temperature: 37°C
Growth Note:
1. Streak out the strain on LB agar containing chloramphenicol (15 µg/ml) to ensure that you start with an F+ host.
2. Do not include chloramphenicol in liquid media: the strain does not grow well.
3. CJ236 is non-suppressing. Do not use M13 vectors that carry amber mutations (mp8, 9 and some mp10 and 11).
4. Store plates at 4°C in the dark.
Strain Properties
dam/dcm Methylation:
dam+
, dcm+
Protease-deficient:
No
Laclq:
No
Drug Resistance: cam
Storage Conditions
Storage Temperature:
-20°C
For long term storage (>30 days), store at -70°C.
Notes
General notes:- CJ236 is dut- ung- and contains an F-factor carrying a selectable marker (chloramphenicol resistance). It was made by taking an isolate of BW313 that had spontaneously become F- and introducing pCJ105, the F´ CmR construct (1,2). For use in preparing uracil-containing M13 templates (3,4).
Usage notes:- Rubidium Chloride Method: RbCl Transformation Protocol
References
- Joyce, C. and Grindley, N. (1984) J. Bacteriol., 158, 636-643.
- Raleigh, E.A., Lech, K. and Brent, R. (1989) F.M. Ausubel et al. (Eds.), Current Protocols in Molecular Biology, pp. x.
- Kunkel, T.A., Bebenek, K. and McClary, J. (1991) J. Miller (Eds.), Methods Enzymol., 204, pp. 125-139. San Diego: Academic Press.
- Kunkel, T.A. et al. (1987) R. Wu and L. Grossman (Eds.), Methods Enzymol., 154, pp. 367-382. San Diego: Academic Press.
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