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E. coli K12 UT5600 |
 | | | | E. coli K12 UT5600 | | | Limit of 1 per order | |
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Description:
A suspension of E. coli K12 UT5600 which has been grown in LB medium and brought to 50% glycerol. This strain is deficient in the OmpT protease, a periplasmic protease. This protease can degrade cytoplasmically expressed proteins when the cells are lysed to make a crude extract, and it may degrade proteins expressed in the periplasm as well. UT5600 is EcoK r+ m+, so plasmids have to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants.
Genotype: F- ara-14 leuB6 secA6 lacY1 proC14 tsx-67 Δ(ompT-fepC)266 entA403 trpE38 rfbD1 rpsL109 xyl-5 mtl-1 thi-1
Recommended Growth Medium: LB
Growth Temperature: 37°C
Strain Properties
dam/dcm Methylation:
dam+
, dcm+
Protease-deficient:
Yes
Laclq:
No
Drug Resistance: str
Storage Conditions
Storage Temperature:
-20°C
For long term storage (>30 days), store at -70°C.
Notes
Usage notes:- Rubidium Chloride Method: RbCl Transformation Protocol
References
- Elish, M.E., Pierce, J.R. and Earhart, C.F. (1988) J. Gen. Microbiol., 134, 1355-1364.
- Grodberg, J. and Dunn, J. (1988) J. Bacteriol., 170, 1245-1253.
- Sugimura, K. and Higashi, N. (1988) J. Bacteriol., 170, 3650-3654.
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