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E. coli K12 KS1000 |
 | | | | E. coli K12 KS1000 | | | Limit of 1 per order | |
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Description:
A suspension of E. coli K12 KS1000 which has been grown in M63 or M9 minimal glucose medium and brought to 50% glycerol. This strain is deficient in the Prc protease, a periplasmic protease (formerly known as Tsp, tail-specific protease). The Prc protease can degrade cytoplasmically expressed proteins when the cells are lysed to make a crude extract, and presumably it can degrade proteins expressed in the periplasm as well.
Genotype: F' lacIq lac+ pro+/ ara Δ(lac-pro) Δ(tsp)= Δ(prc)::KanR eda51::Tn10(TetR) gyrA(NalR) rpoB thi-1 argI(am)
Growth Temperature: 37°C
Growth Note: Grow by streaking out on a glucose minimal and arginine plate and incubating at 37°C.
Strain Properties
dam/dcm Methylation:
dam+
, dcm+
Protease-deficient:
Yes
Laclq:
Yes
Drug Resistance: kan, tet, nal
Storage Conditions
Storage Temperature:
-20°C
For long term storage (>30 days), store at -70°C.
Notes
General notes:- KS1000 is EcoK r+m+, so plasmids have to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants.
Usage notes:- Rubidium Chloride Method: RbCl Transformation Protocol
References
- Silber, K.R., Keiler, K.C. and Sauer, R.T. (1992) Proc. Natl. Acad. Sci. USA, 89, 295-299.
- Miller (1972) x (Eds.), Experiment in molecular genetics, pp. 431-432.
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