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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Strains > Strains > E. coli K12 ER2508 E. coli K12 ER2508 Limit of 1 per order Catalog # Size Concentration Price Qty   E4127S 200 μl   $0.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A suspension of E. coli strain ER2508 which has been grown in LB medium and brought to 50% glycerol. Genotype: F- ara-14 leuB6 fhuA2 Δ(argF-lac)U169 lacY1 lon::miniTn10(TetR) glnV44 galK2 rpsL20(StrR) xyl-5 mtl-5 Δ(malB) zjc::Tn5(KanR) Δ(mcrC-mrr)HB101 Recommended Growth Medium: LB Growth Temperature: 37°C Strain Properties dam/dcm Methylation: dam+ , dcm+ Protease-deficient: Yes Laclq: No Drug Resistance: kan, tet, str Storage Conditions Storage Temperature: -20°C For long term storage (>30 days), store at -70°C. Notes General notes: This strain is a derivative of RR1 that has been made lon-. The lon mutation knocks out the major ATP-dependent protease in the E. coli cytoplasm, thus improving the stability of many foreign proteins when expressed in E. coli. The malE gene, which encodes Maltose-Binding Protein, is deleted as part of the malB deletion. This strain replaces PR745. It is fhuA2. Usage notes: Rubidium Chloride Method: RbCl Transformation Protocol References Kowit, J.D. and Goldberg, A.L. (1977) J. Biol. Chem., 252, 8350-8357. Grossman, A.D., Burgess, W.W. and Gross, C.A. (1983) Cell, 32, 151-159.