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E. coli K12 PR1031 |
 | | | | E. coli K12 PR1031 | | | Limit of 1 per order | |
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Description:
A suspension of E. coli K12 strain PR1031 which has been grown in LB medium and brought to 50% glycerol. Grow by streaking out on an LB plate and incubating at 37°C. This strain is deficient in the DnaJ "chaperonin", and several unstable proteins have been shown to be more stable in this host.
Genotype: F- thr:Tn10(TetR) dnaJ259 leu fhuA2 lacZ90(oc) lacY glnV44 thi
Recommended Growth Medium: LB
Growth Temperature: 37°C
Strain Properties
dam/dcm Methylation:
dam+
, dcm+
Protease-deficient:
Yes
Laclq:
No
Drug Resistance: tet
Storage Conditions
Storage Temperature:
-20°C
For long term storage (>30 days), store at -70°C.
Notes
General notes:- PR1031 is EcoK r+ m+, so your plasmid has to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants.
Usage notes:- Rubidium Chloride Method: RbCl Transformation Protocol
References
- Straus, D.B., Walter, W.A. and Gross, C.A. (1988) Genes and Devel., 2, 1851-1858.
- Reidhaar-Olson, J.F., Parsell, D.A. and Sauer, R.T. (1990) Biochemistry, 29, 7563-7571.
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