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E. coli K12 CAG629 |
 | | | | E. coli K12 CAG629 | | | Limit of 1 per order | |
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Description:
A suspension of E. coli strain K12 CAG629 which has been grown in LB medium and brought to 50% glycerol. E. coli K12 CAG629 has the rpoHam allele (sometimes referred to as htpRam) which encodes an amber mutant form of the sigma factor for the heat shock response. Grow by streaking out on an LB plate and incubating at 30°C.
Genotype: F- lacZ(am) pho(am) lon supC(ts) trp(am) rpsL rpoH(am)165 zhg::Tn10 mal(am)
Recommended Growth Medium: LB
Growth Temperature: 30°C
Strain Properties
dam/dcm Methylation:
dam+
, dcm+
Protease-deficient:
Yes
Laclq:
No
Drug Resistance: tet, str
Storage Conditions
Storage Temperature:
-20°C
For long term storage (>30 days), store at -70°C.
Notes
General notes:- The strain has a temperature-sensitive amber supressor tyrT(supCts), so upon incubation at 37°C or 42°C the strain does not suppress the heat shock sigma factor RpoH. Thus it becomes deficient in expression of the heat shock proteins. Since a number of heat shock proteins affect proteolysis, this can increase the stability of foreign proteins expressed in E. coli. CAG629 is EcoK r+m+, so plasmids have to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants; the transformation frequency is 10-100X down from other common strains used for recombinant DNA work.
Usage notes:- Rubidium Chloride Method: RbCl Transformation Protocol
References
- Baker, T.A., Grossman, A.D. and Gross, C.A. (1984) Proc. Natl. Acad. Sci. USA, 81, 6779-6783.
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