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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Strains > Strains > E. coli K12 CAG626 E. coli K12 CAG626 Limit of 1 per order Catalog # Size Concentration Price Qty   E4124S 200 μl   $0.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A suspension of E. coli K12 CAG626 which has been grown in LB medium and brought to 50% glycerol. Genotype: F- lacZ(am) pho(am) lon trp(am) tyrT[supC(ts)] rpsL(StrR) mal(am) Recommended Growth Medium: LB Growth Temperature: 37°C Strain Properties dam/dcm Methylation: dam+ , dcm+ Protease-deficient: Yes Laclq: No Drug Resistance: str Storage Conditions Storage Temperature: -20°C For long term storage (>30 days), store at -70°C. Notes General notes: CAG626 is EcoK r+m+, so plasmids have to be modified (i.e., come from an m+ strain such as TB1, JM83, JM107, etc.) in order to get transformants; the transformation frequency is 10-100x down from other common strains used for recombinant DNA work. Usage notes: Rubidium Chloride Method: RbCl Transformation Protocol References Grossman, A.D., Burgess, W.W. and Gross, C.A. (1983) Cell, 32, 151-159. Chung, C.H. and Goldberg, A.L. (1981) Proc. Natl. Acad. Sci. USA, 78, 4931-4935.