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E. coli K12 TB1 |
 | | | | E. coli K12 TB1 | | | Limit of 1 per order | |
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Prices are in US dollars and valid only for US orders.
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Description:
A suspension of E. coli strain K12 TB1 which has been grown in LB medium and brought to 50% glycerol. This strain is a derivative of JM83 that has been made hsdR so it will not restrict unmodified DNA.
Genotype: F- ara Δ(lac-proAB) [Φ80dlac Δ(lacZ)M15] rpsL(StrR) thi hsdR
Recommended Growth Medium: LB
Growth Temperature: 37°C
Strain Properties
dam/dcm Methylation:
dam+
, dcm+
Protease-deficient:
No
Laclq:
No
Drug Resistance: str
Storage Conditions
Storage Temperature:
-20°C
For long term storage (>30 days), store at -70°C.
Notes
Usage notes:- Rubidium Chloride Method: RbCl Transformation Protocol
References
- Johnston, T.C. et al. (1986) J. Biol. Chem., 261, 4805-4811.
- Yanisch-Perron, C. et al. (1985) Gene, 33, 103-119.
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