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E. coli K12 ER2925 |
 | | | | E. coli K12 ER2925 | | | Limit of 1 per order | |
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Prices are in US dollars and valid only for US orders.
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Description:
A suspension of E. coli ER2925 which has been grown in L-Broth and brought to 50% glycerol.
Genotype: ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2
Recommended Growth Medium: LB
Growth Temperature: 37°C
Strain Properties
dam/dcm Methylation:
dam-
, dcm-
Protease-deficient:
No
Laclq:
No
Drug Resistance: cam
Storage Conditions
Storage Temperature:
-20°C
For long term storage (>30 days), store at -70°C.
Notes
General notes:- This strain is both Dam- and Dcm- , so it is useful for production of DNA to be cut with Dam or Dcm-sensitive restriction enzymes (1,2). Suppresses many amber mutations when glutamine is acceptable but not the S100 or S7 mutations of λ, e.g., λgt11. Strain is identical to our previous Dam- Dcm- strain GM2163 except that the activity of nonspecific endonuclease I has been abolished, dramatically improving plasmid prep quality in many cases.
- Rubidium Chloride Method: RbCl Transformation Protocol
Usage notes:- Avoid repeated freeze/thaw cycles.
References
- Woodcock, D. M. et al. (1989) Nucl. Acids Res, 17, 3469-3478.
- Palmer, B. R., and Marinus, M. G. (1994) Gene, 143, 1-12.
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