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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Strains > Strains > E. coli K12 NM522 E. coli K12 NM522 Limit of 1 per order Catalog # Size Concentration Price Qty   E4108S 200 μl   $0.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A suspension of E. coli strain NM522 which has been grown in LB medium and brought to 50% glycerol. Genotype: F' proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5 Recommended Growth Medium: LB Growth Temperature: 37°C Strain Properties dam/dcm Methylation: dam+ , dcm+ Protease-deficient: No Laclq: Yes Storage Conditions Storage Temperature: -20°C For long term storage (>30 days), store at -70°C. Notes Usage notes: Rubidium Chloride Method: RbCl Transformation Protocol References Woodcock, D.M. et al. (1989) Nucl. Acids Res., 17, 3469-3478. Gough, J. and Murray, N. (1983) J. Mol. Biol., 166, 1-19.