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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Strains > Strains > E. coli K12 JM109 E. coli K12 JM109 Limit of 1 per order Catalog # Size Concentration Price Qty   E4107S 200 μl   $0.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A suspension of E. coli K12 JM109 which has been grown in minimal media and brought to 50% glycerol. Genotype: F´ traD36 proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV44 e14- gyrA96 recA1 relA1 endA1 thi hsdR17 Recommended Growth Medium: M9 Growth Temperature: 37°C Strain Properties dam/dcm Methylation: dam+ , dcm+ Protease-deficient: No Laclq: Yes Drug Resistance: nal Storage Conditions Storage Temperature: -20°C For long term storage (>30 days), store at -70°C. Notes Usage notes: Rubidium Chloride Method: RbCl Transformation Protocol References Yanish-Perron, C., Vieira, J. and Messing, J. (1985) Gene, 33, 103-119.