 |
|
 |
 |
 |
| Home >
Products >
Strains >
Strains >
E. coli K12 JM101 |
 |  |  | | E. coli K12 JM101 |  |  | Limit of 1 per order |  |
|  |
 |
|
Prices are in US dollars and valid only for US orders.
|

 Description: A suspension of E. coli K12 JM101 which has been grown in minimal media and brought to 50% glycerol.
Genotype: F´ traD36 proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi
Recommended Growth Medium: M9 Growth Temperature: 37°C
Strain Properties

 dam/dcm Methylation:
dam+
, dcm+ Protease-deficient:
No
Laclq:
Yes
Storage Conditions

 Storage Temperature: -20°C For long term storage (>30 days), store at -70°C.
Notes

 Usage notes:- Rubidium Chloride Method: RbCl Transformation Protocol
References


- Messing, J. (1979) A multipurpose cloning system based on the single-stranded DNA bacteriophage M13. Recomb. DNA Tech. Bull. (NIH), 3(2), 43-48.
- Yanish-Perron, C. et al. (1985) Gene, 33, 103-119.
| |
 |
 |
|
 |