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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Strains > Strains > E. coli K12 ER2267 E. coli K12 ER2267 Limit of 1 per order Catalog # Size Concentration Price Qty   E4103S 200 μl   $0.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A suspension of E. coli ER2267 which has been grown in rich medium and brought to 50% glycerol. MM294 background: EcoK r- m- McrA- McrBC- Mrr-. Genotype: F´ proA+B+ lacIq Δ(lacZ)M15 zzf::mini-Tn10 (KanR)/ Δ(argF-lacZ)U169 glnV44 e14-(McrA-) rfbD1? recA1 relA1? endA1 spoT1? thi-1 Δ(mcrC-mrr)114::IS10 Recommended Growth Medium: LB Growth Temperature: 37°C Strain Properties dam/dcm Methylation: dam+ , dcm+ Protease-deficient: No Laclq: Yes Drug Resistance: kan Storage Conditions Storage Temperature: -20°C For long term storage (>30 days), store at -70°C. Notes Restriction Defects: McrA, McrBC, and Mrr all restrict methylcytosine-containing DNA methylated by the CpG methylase (M.Sss I) (1). In addition, McrA and McrBC specifically restrict DNA modified by different sets of more sequence-specific cytosine methylases (3,4). Restriction by each of these three restriction systems individually (tested with λ DNA modified with the Sss I methylase and then packaged into phage) is: McrA, 250-fold; McrBC, 100-fold; Mrr, 200-fold. Cumulative restriction when all three are present in the same cell is 5,000-fold (1). In addition, EcoK restricts DNA not modified at K sites by 10-fold to 5,000-fold, depending on the number of sites (5). ER2267 is deficient in all four systems. Other Properties: Selectable F' (Kan'); suitable for use with M13, pUC-type, and hybrid (phagemid) vectors that make use of lac α-fragment complementation (blue/white screen); overproduces lac repressor (laclq); recA stabilizes repeat sequences and reduces plasmid multimerization. General notes: Selectable F´ (KanR); suitable for use with M13, pUC-type, and hybrid (phagemid) vectors that make use of lac α-fragment complementation (blue/white screen); overproduces lac repressor (lacIq); recA stabilizes repeat sequences and reduces plasmid multimerization. Usage notes: Rubidium Chloride Method: RbCl Transformation Protocol References Kelleher, J.E. and Raleigh, E.A. (1991) J. Bacteriol., 173, 5220-5223. Raleigh, E.A. and Wilson, G. (1986) Proc. Natl. Acad. Sci. USA, 83, 9070-9074. Waite-Rees, P.A. et al. (1991) J. Bacteriol., 173, 5207-5219. Heitman, J. and Model, P. (1987) J. Bacteriol., 169, 3243-3250. Murray, N.E. et al. (1973) Mol. Gen. Genet., 120, 261-281.