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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Strains > Strains > E. coli K12 ER1793 E. coli K12 ER1793 Limit of 1 per order Catalog # Size Concentration Price Qty   E4101S 200 μl   $0.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: A suspension of E. coli ER1793 which has been grown in rich medium and brought to 50% glycerol. ER1370 background; McrA- McrBC- Mrr- EcoK r- m- Genotype: F- fhuA2 Δ(lacZ)r1 glnV44 e14-(McrA-) trp-31 his-1 rpsL104 xyl-7 mtl-2 metB1 Δ(mcrC-mrr)114::IS10 Recommended Growth Medium: LB Growth Temperature: 37°C Strain Properties dam/dcm Methylation: dam+ , dcm+ Protease-deficient: No Laclq: No Drug Resistance: str Storage Conditions Storage Temperature: -20°C For long term storage (>30 days), store at -70°C. Notes General notes: Restriction Defects: McrA, McrBC, and Mrr all restrict methylcytosine-containing DNA methylated by the CpG methylase (M.Sss I) (1). In addition, McrA and McrBC specifically restrict DNA modified by different sets of more sequence-specific cytosine methylases (2). Mrr also restricts many methyladenine-containing sequences (3,4). Restriction by each of these three restriction systems individually (tested with λ DNA modified with the Sss I methylase and then packaged into phage) is: McrA, 250-fold; McrBC, 100-fold; Mrr, 200-fold. Cumulative restriction when all three are present in the same cell is 5,000-fold (1). In addition, EcoK restricts DNA not modified at K sites by 10-fold to 5,000-fold, depending on the number of sites (5). ER1793 is deficient in all four systems (1). This strain was reported to yield good quality libraries of Helicobacter pylori chromosomal DNA (6). Usage notes: Rubidium Chloride Method: RbCl Transformation Protocol References Kelleher, J.E. and Raleigh, E.A. (1991) J. Bacteriol., 173, 5220-5223. Raleigh, E.A. and Wilson, G. (1986) Proc. Natl. Acad. Sci. USA, 83, 9070-9074. Waite-Rees, P.A. et al. (1991) J. Bacteriol., 173, 5207-5219. Heitman, J. and Model, P. (1987) J. Bacteriol., 169, 3243-3250. Murray, N.E. et al. (1973) Mol. Gen. Genet., 120, 261-281. Phadnis, S.H. et al. (1993) Mol. Microbiol., 10, 1151.