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Description:
Description: RheoSwitch® Ligand RSL1 is the transcriptional inducer of the RheoSwitch® Inducible Expression Kit (1). It is specific for the engineered nuclear receptor/activator pair of the RheoSwitch system and can be used to control levels of expression by adjusting its concentration in the culture medium.
RSL1 is the synthetic diacylhydrazine [(N-(2-ethyl-3-methoxybenzoyl)-N´-(3,5-dimethylbenzoyl)-N´-tert-butylhydrazine] (Figure 1). It is one of a family of compounds that have been found to act as non-steroidal ecdysone agonists and can function as gene inducers (2,3).
Figure 1: Chemical structure of RSL1.
Properties & Usage
Molecular Weight:
Theoretical: 382.5 daltons
Reaction & Storage Conditions
Concentration:
5 mM
Storage Conditions:
100% DMSO
Storage Temperature:
-20°C
Notes
Usage notes:- RSL1 can be added directly to culture medium to achieve desired final concentration. For example, 10 µl of RSL1 (5 mM) added to 100 ml of media results in a final concentration of 500 nM.
- Recommended Usage Concentration: RSL1 is active over a broad range of concentration(10 pM to 1 μM). We recommend 500 nM for maximum induction.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
99.4% pure by HPLC.
References
- RheoSwitch Inducible Expression Kit manual., unpublished observations.
- Wing, K.D. (1988) Science, 241, 467-469.
- Dhadialla, T.S., Carlson, G.R. and Le, D.P. (1998) Annu. Rev. Entomol., 43, 545-569.
Companion Products
pNEBR-R1 Regulator Plasmid
pNEBR-X1 Vector
pNEBR-X1GLuc Control Plasmid
pNEBR-X1Hygro Vector
RheoSwitch® Cell Line HEK293-A7
RheoSwitch® Cell Line HEK293-A7 and pNEBR-X1GLuc Control Plasmid
RheoSwitch® Cell Line NIH3T3-47
RheoSwitch® Cell Line NIH3T3-47 and pNEBR-X1GLuc Control Plasmid
RheoSwitch® Mammalian Inducible Expression System
TransPass™ D1 Transfection Reagent
TransPass™ D2 Transfection Reagent
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