 | | | | Gaussia Luciferase Assay Kit |
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Prices are in US dollars and valid only for US orders.
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Description:
The Gaussia Luciferase Assay Kit contains the reagents necessary for assaying Gaussia Luciferase (GLuc) activity, most commonly from cell culture supernatants. Gaussia Luciferase is a new reporter luciferase from the marine copepod Gaussia princeps (1,2). Gaussia Luciferase can be expressed in mammalian cells using reporter plasmids available from NEB. This luciferase, which does not require ATP, catalyzes the oxidation of the substrate coelenterazine in a reaction that emits light (470 nm), and has considerable advantages over other reporter genes.
Figure 1: Kinetics of the GLuc light reaction using the Gaussia Luciferase Assay Kit versus a commercially available Renilla Assay Kit.
Advantages:- Gaussia Luciferase possesses a natural secretory signal and upon expression is secreted into the cell medium. Therefore, lysis of the cells is not necessary.
- Gaussia Luciferase generates over 1000-fold higher bioluminescent signal intensity, compared to Firefly and Renilla Luciferases, making it an ideal transcriptional reporter (2).
- The secreted protein is very stable and can be stored for several days at 4°C without loss of activity.
- The Gaussia Luciferase Assay Buffer has been designed to stabilize the light reaction, which facilitates the assay and gives more consistent results (see Figure 1).
- A simple three step process makes it easy to assay multiple samples and collect data at different time points.
Kit Components:
Gaussia Luciferase Assay Buffer (1X)
Gaussia Luciferase Substrate (100X)
Storage Conditions
Storage Temperature:
-20°C
Notes
General notes:- The Gaussia Luciferase Substrate should be stored in the dark, and tightly capped to prevent evaporation.
- The Gaussia Luciferase Assay Buffer is manufactured by Targeting Systems. The formulation is proprietary.
Usage notes:- One must establish the linear range of the luminometer used for the assay. This is easily done by assaying serial dilutions of an active sample and plotting the results. If excess activity for the instrument range is found, the sample should be diluted in PBS or the integration time may be reduced (e.g. 5 seconds instead of 10 seconds).
Because of the high stability of GLuc, cell supernatants can be stored at -20°C for several weeks with little loss of activity.
FAQs
- Can the GLuc Assay Kit be used for measuring Renilla Luciferase activity?
- Can the GLuc assay working solution be stored after reconstitution?
- Can I add GLuc assay working solution directly to the cells?
- Can the Gaussia Luciferase Assay Kit be used with cell lysates?
References
- Verhaegen M. and Christopoulos T.K. (2002) Anal. Chem., 74, 4378-4385.
- Tannous, B.A., Kim, D.E., Fernandez, J.L., Weissleder, R., and Breakefield, X.O. (2005) Mol. Ther., 11, 435-443.
Companion Products
Luciferase Cell Lysis Buffer
pCMV-GLuc Control Plasmid
pGLuc-Basic Vector
pKLAC1-GLuc Control Plasmid
pNEBR-X1GLuc Control Plasmid
TransPass™ COS/293 Transfection Reagent
TransPass™ D1 Transfection Reagent
TransPass™ D2 Transfection Reagent
TransPass™ HeLa Transfection Reagent
TransPass™ HUVEC Transfection Reagent
TransPass™ V
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