|
|
 |
|
Prices are in US dollars and valid only for US orders.
|
Description:
The Crimson™ Taq PCR Sampler contains sample sizes of recombinant, highly purified Crimson Taq DNA Polymerase, deoxynucleotides and the Quick-Load® 1kb DNA Ladder.
Crimson™ Taq DNA Polymerase offers superior performance in its newly formulated PCR buffer. Crimson Taq Reaction Buffer contains a density reagent, which allows direct loading of PCR products onto a gel. In addition, Crimson Taq Reaction Buffer has trace amounts of a red dye, which serves as an indicator for homogenous reaction setup, a color aid in gel loading and a tracking dye which migrates at about 10 bp on a 1% TBE gel.
The Deoxynucleotide Solution Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP. Each nucleotide is present at a concentration of 10 mM in Milli-Q™ water (pH 7.5 @25°C). The total dNTP concentration equals 40 mM.
The Quick-Load® 1 kb DNA Ladder is a premixed, ready-to-load molecular weight marker containing bromophenol blue as a tracking dye.
Applications:- Primer Extension
- Routine PCR
- Colony PCR
- DHPLC
- Microarray Analysis
- High-throughput PCR
Advantages:- Robust and reliable reactions
- Reactions can be loaded directly on a gel
- Ideal for high throughput applications
Kit Components:
Crimson Taq™ DNA Polymerase
Crimson Taq™ Reaction Buffer Pack (5X)
Deoxynucleotide Solution Mix (10 mM)
Quick-Load® 1 kb DNA Ladder
Storage Conditions
Storage Temperature:
-20°C
FAQs
- What are the advantages or disadvantages of Crimson Taq DNA Polymerase?
- Does the 5X Crimson Taq Reaction Buffer offer amplification efficiency similar to that of Standard Taq Reaction Buffer or ThermoPol Reaction Buffer?
- Can the PCR product be used directly in T/A cloning?
- How do I remove the dye and Dextran from my PCR reactions using Crimson Taq Reaction Buffer?
- How long is the buffer stable in non-optimal storage conditions?
Amplification of specific sequences from human genomic DNA using Crimson Taq DNA Polymerase. Amplicon sizes are indicated below gel. Marker M is NEB 1 kb DNA Ladder (NEB #N3232).
References
- Barnes, W.M. (1994) Proc. Natl. Acad. Sci, 91, 2216-2220.
- Foord, O.S. and Rose, E.A. (1994) PCR Methods Appl., 3, 149-161.
- Sun, Y. et al. (1993) Biotechniques, 15, 372-374.
- Sarkar, G. et al. (1990) Nucleic Acids Res, 18, 7465.
Reagents Sold Separately
Crimson Taq™ DNA Polymerase
Crimson Taq™ Reaction Buffer Pack
Deoxynucleotide Solution Mix
Quick-Load® 1 kb DNA Ladder
Companion Products
Crimson Taq™ (Mg-free) Reaction Buffer Pack
Crimson Taq™ DNA Polymerase
Crimson Taq™ DNA Polymerase with (Mg-free) Buffer
Crimson Taq™ Reaction Buffer Pack
Deoxynucleotide Solution Mix
Quick-Load® 1 kb DNA Ladder
Legal
Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
|
