To access your account, log in or register.	shopping cart | log in

RELATED INFORMATION Protocols for BioBrick™ Assembly Kit FAQs for Restriction Endonucleases Technical Reference for Restriction Endonucleases FAVORITE TOOLS Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE RELATED PRODUCTS Reagents Sold Separately BSA EcoRI-HF™ NEBuffer 2 PstI SpeI T4 DNA Ligase XbaI

Home > Products > Restriction Endonucleases > Synthetic Biology > BioBrick™ Assembly Kit BioBrick™ Assembly Kit Catalog # Size Concentration Price Qty   E0546S 50 reactions   $235.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: The BioBrick™ Assembly Kit provides a streamlined method for assembly of BioBrick™ parts into multi-component genetic systems. BioBrick™ parts are DNA sequences that encode a defined biological function and can be readily assembled with any other BioBrick™ part. The process for assembling any two BioBrick™ parts is identical and results in a new composite BioBrick™ part. The BioBrick™ Assembly Kit was developed in partnership with Ginkgo BioWorks. For more details and for technical questions, please see: http://ginkgobioworks.com/support. Assembly strategy for two BioBrick™ standard biological parts. The Upstream Part is digested with EcoRI-HF and SpeI. The Downstream Part is digested with XbaI and PstI. The Destination Plasmid into which the two parts will be assembled is digested with EcoRI-HF and PstI. The three fragments are mixed, incubated with T4 DNA Ligase and transformed into E. coli. The desired composite BioBrick™ part is selected for by plating on LB agar plates supplemented with the antibiotic corresponding to the Destination Plasmid resistance marker. Kit Components: BSA EcoRI-HF™ NEBuffer 2 PstI SpeI T4 DNA Ligase XbaI Protocols Protocols for BioBrick™ Assembly Kit Reagents Sold Separately BSA EcoRI-HF™ NEBuffer 2 PstI SpeI T4 DNA Ligase XbaI Legal Licenses/Patents/Disclaimers: BioBrick™ is a trademark of The BioBricks Foundation. (http://www.biobricks.org)