To access your account, log in or register.	shopping cart | log in

RELATED INFORMATION FAQs for K. lactis YCT598 Competent Cells Protocols for K. lactis YCT598 Competent Cells FAQs for Protein Expression and Purification Technical Reference for Protein Expression and Purification FAVORITE TOOLS Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE

Home > Products > Protein Expression and Purification > Yeast > K. lactis YCT598 Competent Cells K. lactis YCT598 Competent Cells Catalog # Size Concentration Price Qty   C1006S 5 transformation reactions   $290.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: Chemically competent Kluyveromyces lactis YCT598 cells are a suitable host for expressing proteins that have exhibited proteolysis in the parental GG799 background (NEB #C1001). YCT598 cells can be transformed with any linearized pKLAC-series or pKLMF-series expression vector. K. lactis strain YCT598 carries a selectable marker-free deletion of the KLLA0D15917g locus encoding an extracellular aspartyl protease that is homologous to S. cerevisiae Bar1p. Strain: K. lactis strain YCT598 (Δ KLLA0D15917g). No auxotrophies or genetic markers. Reagents Supplied with Cells: NEB Yeast Transformation Reagent (NEB #M2570). Store at 4°C. Storage Conditions Storage Temperature: -80°C Notes General notes: Store Competent Cells at -80°C. Once Thawed, Do Not Re-freeze Usage notes: Due to the high transformation efficiency of K. lactis YCT598 Competent Cells, plating multiple dilutions of the cell mixture is necessary to ensure formation of plates with distinct single colonies. Growth time should not exceed 5 days as small colonies that lack an integrated expression fragment may form. Plates containing colonies can be stored at 4°C for up to two weeks. Growth of K. lactis YCT598 Competent Cells carrying an integrated expression vector for protein expression should be performed in YPGal medium (1% yeast extract, 2% peptone, 2% galactose) supplemented with a final concentration of 38 mM (NH4)2SO4. FAQs Why does NEB offer K. lactis protease deletion strains? What K. lactis protease deletion strains does NEB sell? Which protease deletion strain should i use? What vectors may be used with protease deletion strains? What is the parent background for the protease deletion strains? Protocols Protocols for K. lactis YCT598 Competent Cells Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. Competency: One microgram of linearized pKLAC1-malE was used to transform one tube of K. lactis YCT598 Competent Cells following the protocol provided. Greater than 1 x 104 colonies formed after a 3 day incubation at 30°C. Use of cells beyond the expiration date may result in lower transformation efficiency. Sterility: One tube of competent cells and 100 µl NEB Yeast Transformation Reagent were spread onto individual YCB Agar Medium plates containing 5 mM acetamide and incubated at 30°C for 3 days. No bacterial or fungal growth was detected. Legal Licenses/Patents/Disclaimers: Notice to Buyer/User: K. lactis Competent Cells are a component of an expression system that was developed from basic research at New England Biolabs and DSM Biologics Company B.V. The buyer and user has a non-exclusive sublicense to use this system or any component thereof, including the K. lactis GG799, YCT284, YCT569, YCT389, YCT390 and YCT598 Competent Cells, for RESEARCH PURPOSES ONLY. A license to use this system for manufacture of clinical grade material or commercial purposes is available from New England Biolabs, Inc., or DSM Biologics Company B.V.