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K. lactis GG799 Competent Cells |
 | | | | K. lactis GG799 Competent Cells | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
Chemically competent Kluyveromyces lactis cells suitable for transformation with a linearized pKLAC1-based expression vector.
Strain: K. lactis strain GG799. No auxotrophies or genetic markers.
Reagents Supplied with Cells: NEB Yeast Transformation Reagent (Store at 4°C).
Storage Conditions
Storage Temperature:
-80°C
Notes
General notes:- Store Competent Cells at -80°C. Once Thawed, Do Not Re-freeze.
Usage notes:- Due to the high transformation efficiency of K. lactis GG799 Competent Cells, plating multiple dilutions of the cell mixture is necessary to ensure formation of plates with distinct single colonies. Growth time should not exceed 5 days as small colonies that lack an integrated expression fragment may form.
Plates containing colonies can be stored at 4°C for up to two weeks.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Competency:
One microgram of linearized pKLAC1-malE was used to transform one tube of K. lactis GG799 Competent Cells following the protocol provided. Greater than 1 x 104 colonies formed after a 3 day incubation at 30°C.
Use of cells beyond the expiration date may result in lower transformation efficiency.
Sterility:
One tube of competent cells and 100 µl NEB Yeast Transformation Reagent were spread onto individual YCB Agar Medium plates containing 5 mM acetamide and incubated at 30°C for 3 days. No bacterial or fungal growth was detected.
Companion Products
K. lactis Protein Expression Kit
pKLAC1 Vector
pKLAC1-malE Control Plasmid
Yeast Medium Pack
Legal
Licenses/Patents/Disclaimers:
Notice to Buyer/User: K. lactis Competent Cells are a component of an expression system that was developed from basic research at New England Biolabs and DSM Biologics Company B.V. The buyer and user has a non-exclusive sublicense to use this system or any component thereof, including the K. lactis GG799, YCT284, YCT569, YCT389, YCT390 and YCT598 Competent Cells, for RESEARCH PURPOSES ONLY. A license to use this system for manufacture of clinical grade material or commercial purposes is available from New England Biolabs, Inc., or DSM Biologics Company B.V.
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