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FAQs for K. lactis GG799 Competent Cells Protocols for K. lactis GG799 Competent Cells FAQs for Protein Expression and Purification Technical Reference for Protein Expression and Purification Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder PCR Selection Tool Isoschizomers DNA Sequences and Maps REBASE K. lactis Protein Expression Kit pKLAC1 Vector pKLAC1-malE Control Plasmid Yeast Medium Pack

Home > Products > Protein Expression and Purification > Yeast > K. lactis GG799 Competent Cells K. lactis GG799 Competent Cells Catalog # Size Concentration Price Qty   C1001S 5 transformation reactions   $245.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: Chemically competent Kluyveromyces lactis cells suitable for transformation with a linearized pKLAC1-based expression vector. Strain: K. lactis strain GG799. No auxotrophies or genetic markers. Reagents Supplied with Cells: NEB Yeast Transformation Reagent (Store at 4°C). Storage Conditions Storage Temperature: -80°C Notes General notes: Store Competent Cells at -80°C. Once Thawed, Do Not Re-freeze. Usage notes: Due to the high transformation efficiency of K. lactis GG799 Competent Cells, plating multiple dilutions of the cell mixture is necessary to ensure formation of plates with distinct single colonies. Growth time should not exceed 5 days as small colonies that lack an integrated expression fragment may form. Plates containing colonies can be stored at 4°C for up to two weeks. FAQs Why does NEB offer K. lactis protease deletion strains? What K. lactis protease deletion strains does NEB sell? Which protease deletion strain should i use? What vectors may be used with protease deletion strains? What is the parent background for the protease deletion strains? Protocols Protocols for K. lactis GG799 Competent Cells Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. Competency: One microgram of linearized pKLAC1-malE was used to transform one tube of K. lactis GG799 Competent Cells following the protocol provided. Greater than 1 x 104 colonies formed after a 3 day incubation at 30°C.  Use of cells beyond the expiration date may result in lower transformation efficiency. Sterility: One tube of competent cells and 100 µl NEB Yeast Transformation Reagent were spread onto individual YCB Agar Medium plates containing 5 mM acetamide and incubated at 30°C for 3 days. No bacterial or fungal growth was detected. Companion Products K. lactis Protein Expression Kit pKLAC1 Vector pKLAC1-malE Control Plasmid Yeast Medium Pack Legal Licenses/Patents/Disclaimers: Notice to Buyer/User: K. lactis Competent Cells are a component of an expression system that was developed from basic research at New England Biolabs and DSM Biologics Company B.V. The buyer and user has a non-exclusive sublicense to use this system or any component thereof, including the K. lactis GG799, YCT284, YCT569, YCT389, YCT390 and YCT598 Competent Cells, for RESEARCH PURPOSES ONLY. A license to use this system for manufacture of clinical grade material or commercial purposes is available from New England Biolabs, Inc., or DSM Biologics Company B.V. New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201