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Protocols for Yeast Medium Pack FAQs for Buffers Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE K. lactis GG799 Competent Cells K. lactis Protein Expression Kit pKLAC1 Vector

Home > Products > Buffers > Buffers and Supplements > Yeast Medium Pack Yeast Medium Pack Catalog # Size Concentration Price Qty   B9017S 12 grams   $25.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: This medium pack includes 12 grams of Yeast Carbon Base and 10 ml of Acetamide. The Yeast Carbon Base (YCB) Medium Pack contains sufficient reagents needed to make 1 liter of YCB Agar Medium containing 5 mM acetamide. YCB Agar Medium is used for acetamide selection of K. lactis cells that have been transformed with a pKLAC1-based expression vector (NEB #N3740). The medium contains glucose and all nutrients needed to sustain growth of K. lactis GG799 cells except a simple nitrogen source. Only transformed cells can utilize acetamide provided in the medium as a source of nitrogen after it is broken down to ammonia by acetamidase (the product of the amdS gene present in pKLAC1). Reagents Supplied: Yeast Carbon Base Medium 12 grams 100X acetamide solution (sterile) 10 ml Reaction & Storage Conditions Storage Temperature: 4°C Companion Products K. lactis GG799 Competent Cells K. lactis Protein Expression Kit pKLAC1 Vector