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RNAPol Reaction Buffer |
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Prices are in US dollars and valid only for US orders.
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Description:
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
Reagents Supplied:
10X RNAPol Reaction Buffer
1X RNAPol Reaction Buffer:
40 mM Tris-HCl
6 mM MgCl2
10 mM Dithiothreitol
2 mM spermidine
pH 7.9 @ 25°C
Storage Conditions
Storage Temperature:
-20°C
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
16-Hour Incubation:
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.
Endonuclease Activity:
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.
Companion Products
SP6 RNA Polymerase
T7 RNA Polymerase
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