Description: S-adenosyl-methionine (SAM) is included with all of the DNA methylases available from New England Biolabs, Inc. As well as certain restriction enzymes for which it is required. The SAM is prepared in a solution of 0.005 M H2SO4 and 10% ETOH and filtered.
Reagents Supplied: S-adenosylmethionine (SAM)
Storage Conditions Storage Temperature: -20°C
Notes General notes:
For use of methylation reaction the SAM should be diluted 1:400 to a final concentration of 80μM.
Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement: Each lot of SAM is tested in an SssI (CpG) Methylase (NEB #M0226) reaction with l DNA. The DNA is then challenged with BstUI to confirm methylation.