Description: Bovine Serum Albumin (BSA) is supplied with some restriction enzymes to prevent adhesion of the enzyme to reaction tubes and pipette surfaces. BSA also stabilizes some proteins during incubation.
Reagents Supplied: Bovine Serum Albumin
Storage Conditions Storage Conditions: 20 mM KPO4 50 mM NaCl 0.1 mM EDTA 5% Glycerol
pH 7.0 @ 25°C
Storage Temperature: -20°C
Notes General notes:
We do not recommend using this preparation of BSA as a standard for quantitation purposes or size determination.
Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product.
16-Hour Incubation:
A 50 μl reaction containing 1 μg of ΦX174 DNA
and 50 µg of BSA incubated for 16 hours at 37ºC
resulted in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis.
RNase Assay: Incubation of 50 μg of BSA with 1 μg NEB's RNA Ladder (NEB #N0362) for 1 hour at 37°C in 50 μl assay buffer [70 mM Tris-HCI, 10 mM MgCl2, 5 mM DTT, (pH 7.6 @25°C)] followed by agarose gel electrophoresis resulted in no detectable RNA degradation.
Endonuclease Assay: Incubation of 50 μg of BSA with 1 μg ΦX174 RF I DNA for 4 hours at 37°C in 50 μl of 1X NEBuffer 2 resulted in < 10% conversion to RF II.
