Description: Diluent B is recommended for making dilutions of New England Biolabs, Inc. restriction enzymes.
When necessary, we recommend diluting enzymes just prior to use and suggest that the final concentration of diluted enzymes be at least 1,000 units/ml. Restriction enzymes should be stored at their selling concentrations.
Reagents Supplied: 1X Diluent B
1X Diluent B: 300 mM NaCl 10 mM Tris-HCl 1 mM Dithiothreitol 0.1 mM EDTA 500 µg/ml BSA 50 % Glycerol
pH 7.4 @ 25°C
Storage Conditions Storage Temperature: -20°C
Notes General notes:
These enzyme diluent buffers are not for use with VentR® DNA Polymerases. For diluting VentR® DNA Polymerases, use (NEB #B8004S).
