Description: Blue Loading Buffer Reagents for SDS-PAGE:
3X SDS Blue Loading Buffer (8 ml): 187.5 mM Tris-HCl (pH 6.8 @ 25°C), 6% (w/v) SDS, 30% glycerol and 0.03% (w/v) bromophenol blue. (Store at room temperature)
30X Reducing Agent (1 ml): 1.25 M DTT (Store at -20°C)
Storage Conditions Storage Temperature: -20°C
Notes General notes:
Reagents stored at different temperatures.
Usage notes:
Prepare fresh 3X Reducing Blue Loading Buffer by adding 1/10 volume 30X Reducing Agent to 1 volume of 3X Blue Loading Buffer.
Prepare samples by adding 2 volumes of Sample to 1 volume 3X Reducing Blue Loading Buffer (from step 1).
Heat samples to 95-100°C for 3-5 minutes.
After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobiity, load an equal volume of 1X Reducing Blue Loading Buffer into any unused wells.
