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Gel Loading Dye, Blue (6X) |
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Prices are in US dollars and valid only for US orders.
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Description:
Gel Loading Dye, Blue (6X) is a pre-mixed loading buffer with one tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue is the standard tracking dye for electrophoresis. It migrates at approximately 300 bp on a standard 1% TBE agarose gel.
Reagents Supplied:
Gel Loading Dye, Blue (6X)
1X Gel Loading Dye, Blue (6X):
2.5 % Ficoll 400
11 mM EDTA
3.3 mM Tris-HCl
0.017 % SDS
0.015 % Bromophenol Blue
pH 8.0 @ 25°C
Storage Conditions
Storage Temperature:
25°C
Notes
Usage notes:- Use 5 µl of Gel Loading Dye, Blue (6X) per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
Quality Assurance Statement:
Gel Loading Dye, Blue (6X) is assayed for contaminating endonuclease, exonuclease and RNase activity.
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