Description: New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer.
Restriction endonucleases that do not require BSA for optimal activity are not adversely affected if BSA is present in the reaction mixture.
Reagents Supplied: 10X NEBuffer BsrFI
1X NEBuffer BsrFI: 50 mM NaCl 100 mM Tris-HCl 10 mM MgCl2 0.025 % Triton X-100
pH 7.5 @ 25°C
