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Home > Products > Buffers > Buffers and Supplements > Terminal Transferase Reaction Buffer Pack Terminal Transferase Reaction Buffer Pack Catalog # Size Concentration Price Qty   B0315S 6 ml   $14.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme. Reagents Supplied: 10X Terminal Transferase Reaction Buffer 2 x 1.5 ml 10X CoCl2 (2.5 mM) 2 x 1.5 ml Reaction Conditions:  At a 1X concentration the reaction buffer, supplemented with 0.25 mM CoCl2 assures optimal activity for Terminal Transferase. 1X Terminal Transferase Reaction Buffer: 50 mM potassium acetate 20 mM Tris-acetate 10 mM magnesium acetate pH 7.9 @ 25°C Supplied as a 10X concentrated stock. Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. 16-Hour Incubation: A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested φX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation. Endonuclease Activity: Incubation of this reaction buffer at a 1X concentration with 1 µg φX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II. New England Biolabs, Inc. is an ISO 9001 and ISO 14001 Certified Company. NEB certifies that it is a small business in accordance with the US Small Business Administration and 13 CFR 121.201