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Enzyme Finder NEBcutter NEBuffer Chart Double Digest Finder Isoschizomers DNA Sequences and Maps REBASE Endonuclease III (Nth)

Home > Products > Buffers > Buffers and Supplements > Endonuclease III (Nth) Reaction Buffer Endonuclease III (Nth) Reaction Buffer Catalog # Size Concentration Price Qty   B0268S 6 ml   $10.00 Prices are in US dollars and valid only for US orders. Download: MSDS PDF Reagents Supplied: 10X Endonuclease III (Nth) Reaction Buffer 1X Endonuclease III (Nth) Reaction Buffer: 20 mM Tris-HCl 1 mM EDTA 1 mM Dithiothreitol pH 8.0 @ 25°C Storage Conditions Storage Temperature: -20°C Quality Control for Current Lot Quality control values for a specific lot can be found on the datacard which accompanies each product. 16-Hour Incubation:: A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation. Endonuclease Activity:: Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II. Companion Products Endonuclease III (Nth)