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Mung Bean Nuclease Reaction Buffer |
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Prices are in US dollars and valid only for US orders.
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 Description: New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
Reagents Supplied: 10X Mung Bean Nuclease Reaction Buffer
1X Mung Bean Nuclease Reaction Buffer: 50 mM sodium acetate 30 mM NaCl 1 mM ZnSO4
pH 5.0 @ 25°C
Storage Conditions

 Storage Temperature: -20°C
Notes

 Usage notes:- Also active in all four NEBuffers.
FAQs


- What is supplied with the Mung Bean Nuclease Reaction Buffer pack?
- What is the composition of Mung Bean Nuclease Buffer?
- How should the NEBuffer be used?
- The buffer arrived thawed; is it and the enzyme still active?
Quality Control for Current Lot

 Quality control values for a specific lot can be found on the datacard which accompanies each product.
16-Hour Incubation:: A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.
Endonuclease Activity:: Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.
Companion Products

 Mung Bean Nuclease
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