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Mung Bean Nuclease
Mung Bean Nuclease Reaction Buffer
Catalog # Size Concentration Price Qty  
B0250S 6 ml   $10.00
Prices are in US dollars and valid only for US orders.
Download:MSDS PDF


Description:
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.


Reagents Supplied:
10X Mung Bean Nuclease Reaction Buffer

1X Mung Bean Nuclease Reaction Buffer:
50 mM sodium acetate
30 mM NaCl
1 mM ZnSO4
pH 5.0 @ 25°C


Storage Conditions


Storage Temperature:
-20°C


Notes


Usage notes:
  1. Also active in all four NEBuffers.

FAQs


  1. What is supplied with the Mung Bean Nuclease Reaction Buffer pack?
  2. What is the composition of Mung Bean Nuclease Buffer?
  3. How should the NEBuffer be used?
  4. The buffer arrived thawed; is it and the enzyme still active?

Quality Control for Current Lot


Quality control values for a specific lot can be found on the datacard which accompanies each product.

16-Hour Incubation::
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.

Endonuclease Activity::
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.


Companion Products


Mung Bean Nuclease

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