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dam Methyltransferase Reaction Buffer |
 | | | | dam Methyltransferase Reaction Buffer | | | |
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Prices are in US dollars and valid only for US orders.
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Description:
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. The 1X buffer for dam Methyltransferase ensures optimal activity of the enzyme.
Reagents Supplied:
10X dam Methyltransferase Reaction Buffer
1X dam Methyltransferase Reaction Buffer:
50 mM Tris-HCl
10 mM EDTA
5 mM 2-Mercaptoethanol
pH 7.5 @ 25°C
Storage Conditions
Storage Temperature:
-20°C
Quality Control for Current Lot
Quality control values for a specific lot can be found on the datacard which accompanies each product.
16-Hour Incubation::
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.
Endonuclease Activity::
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.
Companion Products
dam Methyltransferase
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